학술논문
A Second Form of Phenylalanine Ammonia-Lyase From Leaf Mustard
이용수 3
- 영문명
- A Second Form of Phenylalanine Ammonia-Lyase From Leaf Mustard
- 발행기관
- 강원대학교 기초과학연구소
- 저자명
- Hye-Won Lim Jae-Hoon Sa Soo-Sun Park Chang-Jin Lim
- 간행물 정보
- 『기초과학연구』제9집, 230~236쪽, 전체 7쪽
- 주제분류
- 자연과학 > 자연과학일반
- 파일형태
- 발행일자
- 1998.12.01
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국문 초록
영문 초록
Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) catalyzes the elimination of ammonium ion from L-Phenylalanine in a variety of plants and fungal species. PAL was previously purified and characterized from leaf mustard in our laboratory. In the present study, we purified a second phenylalanine ammonia-lyase (PAL II) from leaf mustard by a combination of ion exchange chromatography and gel filtration. PAL I and PAL II migrate at a different rate on native polyacrylamide gel electrophoresis. It consists of four subunits, each having the molecular mass of about 37,000 Da. Its isoelectric point and Km value for L-phenylalanine were found to be 5.4 and 3.8×10??M, respectively. The purified enzyme has an optimum pH and temperature of 8 and 45℃, respectively. It is activated about 2-fold by caffeic acid (1 mM), whereas it is inhibited to 15％ by Zn²??(1 mM). However, the physiological role of PAL II remains unknown.
목차
Introduction
Materials and Methods
Results and Discussion
References
키워드
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