학술논문
벼(O.sativa)의 하배축 절편체로부터 유도된 배발생캘러스와 현탁배양세포의 특성
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- 영문명
- Embryogenic Callus and Suspenson-Cultured Cells induced from Rice(O.sativa) Hypocotyl Explants
- 발행기관
- 강원대학교 기초과학연구소
- 저자명
- 김준철(Joon C. KIM) 김명덕(Myoung D. Kim) 손수인(Su I. Son)
- 간행물 정보
- 『기초과학연구』제9집, 143~155쪽, 전체 13쪽
- 주제분류
- 자연과학 > 자연과학일반
- 파일형태
- 발행일자
- 1998.12.01
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국문 초록
영문 초록
Frequency of germination and contamination from the seeds of 13 rice (O. sativa) cultivars was investigated. Also frequency of callus induction from the hypocotyl explants of seedling was tested on LS medium supplemented with 2.5 mg/L 2,4-D in the dark at 27℃. Hypocotyl explants of selected 4 rice (O. sativa) cultivars were cultured on LS medium supplemented with various concentrations of 2,4-D. The highest frequency of callus formation was obtained when cv. Koshihikari, cv. Nakdong, cv. Ginbuchal and cv. Ricol were cultured on medium supplemented with 2.5 mg/L, 1.0 mg/L, 1.5 mg/L and 2.0 mg/L 2,4-D respectively. Embryogenic calli of pale yellow, globular type were selected and cultured in AA₂ medium supplemented with 2.0 mg/L 2,4-D, 0.2 mg/L kinetin and 0.1 mg/L GA₃ for suspension culture. Initial suspension culture showed large elongated cells and round cells with granular cytoplasm. The fine suspension cell lines were established after 2 months of subculture.
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