Physiological and morpho-anatomical analyses of hyperhydric Arabidopsis thaliana influenced by media components

• 발행기관: (사)한국식물생명공학회
• 저자명: Kemat Nurashikin Visser Richard G.F. Krens Frans
• 간행물 정보: 『Journal of Plant Biotechnology』50권 4호, 255~266쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.12.31

국문 초록

Hyperhydricity is a physiological anomaly that significantly affects the growth and proliferation rate of crops cultivated by tissue culture techniques. To better understand the mechanisms that govern hyperhydricity incidence, we examined the effects of several media components, particularly cytokinin and gelling agents. These elements were found to be influential in both in vitro propagation and the development of hyperhydricity. Our study revealed that Arabidopsis thaliana seedlings had a greater manifestation of hyperhydricity symptoms when exposed to high cytokinin concentrations compared with the control. The presence of gelrite led to the manifestation of hyperhydric symptoms by elevated water build-up in the apoplast. The phenomenon of stomata closure was observed in the hyperhydric leaves, resulting in an increased ability to retain water and a decrease in the transpiration rates when compared to their respective control leaves. Additionally, histological examinations of the cross sections of hyper-hydric leaves revealed an irregular cellular arrangement and large intercellular spaces. Furthermore, hyperhydric seedlings displayed impaired cuticular development in comparison to their normal seedlings.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Acknowledgement

References

키워드

Apoplast Cytokinin Gelling agents Hyperhydricity In vitro culture Micropropagation