‘다유들깨’품종의 하배축에서 캘러스를 통한 고효율 식물재분화

• 영문명: Efficient plant regeneration through callus induction from the hypocotyl of Perilla frutescens L var. Dayu
• 발행기관: (사)한국식물생명공학회
• 저자명: 서여월 손지희 강홍규 선현진 이효연
• 간행물 정보: 『Journal of Plant Biotechnology』50권 4호, 248~254쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.12.31

국문 초록

This study was conducted to establish an efficient plant regeneration system in ‘Dayu’, a Korean variety of Perilla frutescens developed for seed oil production, in conjunction with the previously studied variety ‘Namcheon’. The healthiest callus was formed on the hypocotyl explants cultured on a medium containing 0.1 mg/L NAA and 0.5 mg/L BA, outperforming the leaf and cotyledon samples. In both dark and long-day conditions, Dayu consistently exhibited significantly higher shoot regeneration rates compared with Namcheon. The highest shoot regeneration rates in Dayu were observed from the hypocotyl explants cultured on 0.1 mg/L NAA and 0.5 mg/L BA media, with shoot regeneration rates of 84.4% and 86.7% under dark and long-day conditions, respectively. Various combinations of plant growth regulators were tested to establish the optimal shoot regeneration conditions for Dayu hypocotyl explants. The results demonstrated that the highest shoot regeneration rate (90%) was achieved when 0.5 mg/L of BA was added to the medium without NAA. Among the regenerated shoots, 70.5% were normal plants, while 19.3% were abnormal. The addition of NAA or an increase in its concentration led to a higher occurrence of abnormal plants. After the regenerated shoots were transferred to 1/2 MS medium, roots were observed within 10-15 days. By day 30, they had developed into complete plants. The results obtained from the regenera- tion experiments with the perilla variety Dayu can valuably inform molecular breeding reliant on transformation tech- niques such as genome-editing and genetic modification technology.

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키워드

Perilla frutescens Korean perilla Dayu variety Hypocotyl Callus induction Plant regeneration