국화 ‘오블랑’의 재생 효율 증진을 위한 식물생장조절제와 AgNO₃ 적정 농도 선별

• 영문명: Optimal concentrations of plant growth regulators and AgNO₃ for the improvement of regeneration efficiency in Chrysanthemum morifolium ‘Ohblang’
• 발행기관: (사)한국식물생명공학회
• 저자명: 윤여진 양용준
• 간행물 정보: 『Journal of Plant Biotechnology』50권 4호, 169~175쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2023.12.31

국문 초록

A plant regeneration system was developed through shoot organogenesis from in vitro leaf explants of Chrysanthemum morifolium ‘Ohblang’. The effects of different concentrations of plant growth regulators and AgNO3 on efficient shoot regeneration and inhibition of browning were evaluated in chrysanthemum. The explants were cultured on MS shoot induction medium supplemented with 12 combination treatments of 6-benzyladenine (BA) 0.5, 1.0 and 2.0 mg/L, and α-naphthaleneacetic acid (NAA) 0.2, 0.5, 1.0 and 2.0 mg/L in darkness for 6 weeks and cultured under a 16/8 h photoperiod for 6 weeks. The highest shoot regeneration was obtained from the explants cultured on the medium with 1.0 mg/L BA and 1.0 mg/L NAA. Based on this result, AgNO3 was added to a shoot induction medium containing MS salts, vitamins, 1.0 mg/L BA, 1.0 mg/L NAA, 30 g/L sucrose, and 6 g/L agar to reduce browning of chrysanthemum leaf explants. In the control treatment without AgNO3, leaf explants turned brown at the cut edge; however, browning was not observed in AgNO3 treatments. Shoot organogenesis was higher at low concentrations of AgNO3 and decreased with an increase in AgNO3 concentration. The explants cultured on shoot induction medium (MS salts, vitamins, 1.0 mg/L BA, 1.0 mg/L NAA) with 1 mg/L of AgNO3 produced the highest shoot regeneration with 2.6 shoots per explants and a browning index of 0.7. When the regenerated shoots were detached from the explants and cultured on MS medium, the shoots were elongated and rooted successfully.

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키워드

Chrysanthemum ‘Ohblang’, Shoot organogenesis Plant regeneration Silver nitrate