Agrobacterium을 이용한 형질전환 벼 현탁 세포주에서 miraculin 단백질의 생산

• 영문명: Production of miraculin protein in suspension cell lines of transgenic rice using Agrobacterium
• 발행기관: (사)한국식물생명공학회
• 저자명: 김희경(Hee Kyoung Kim) 고지윤(Ji Yun Go) 박소영(So-Young Park) 강권규(Kwon Kyoo Kang) 정유진(Yu Jin Jung)
• 간행물 정보: 『Journal of Plant Biotechnology』47권 3호, 227~234쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2020.09.30

영문 초록

To produce the miraculin protein in suspension cultures, rice (Oryza sativa L.) was transformed with Agrobacterium tumefacience EHA105 containing the miraculin AB512278 gene. The cell suspension cultures were established using cell lines selected from transgenic rice callus. The integration of the miraculin gene into the rice chromosome was confirmed using genomic PCR analysis. In addition, RT-PCR analysis indicated that the miraculin gene is expressed in the selected suspension cell lines. Thus, the recombinant miraculin was expressed in the transgenic suspension cell line, HK-2. Therefore, we have successfully developed a HK-2 line that produces miraculin. These results demonstrate that transformed cell suspension cultures can be used to produce a taste-modifying protein such as miraculin.

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키워드

Cell suspension culture Rice transformation Miraculin Taste-modifying protein