Micropropagation from root segments to improve seedling quality in Chinese foxglove crops

• 발행기관: (사)한국식물생명공학회
• 저자명: Thanh Loan Pham Van Huy Nguyen Thi Le Thu Hoang Thi Tam Tien Ha Trung Kien Tran Xuan Duong Vu Phi Ba
• 간행물 정보: 『Journal of Plant Biotechnology』47권 3호, 235~241쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2020.09.30

영문 초록

This is the first study to establish a complete protocol for micropropagation of Rehmannia glutinosa from root segments. The study involved investigating the effect of plant growth regulators on in vitro shoot regeneration and rooting and identifying substrates supporting survival and growth performance of ex vitro seedlings. A Murashige and Skoog (MS) medium containing 30 g/L sucrose for shoot induction and 0.2 mg/L indole-3-acetic acid (IAA), 1 mg/L 6-benzylaminopurine (BAP), and 1 g/L polyvinylpyrrolidone (PVP) for shoot multiplication resulted in the highest number of shoots per explant and shoot height. Applying a medium containing 0.5 mg/L IAA and 1 g/L PVP yielded optimal rooting of the shoots grown in vitro. Compost enriched with microbial inoculants and perlite enhanced seedling growth better than that with organic biofertilizer-free substrates (soil and sand). We recommend the continuous production of micropropagated R. glutinosa seedlings from root segments under the aforementioned conditions as a possible propagation technique for crops of this species.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

Acknowledgments

Reference

키워드

IAA Multiple shoots NAA Rehmannia PVP Root induction Tissue culture