Improved plastid transformation efficiency in Scoparia dulcis L.

• 발행기관: (사)한국식물생명공학회
• 저자명: Srinivas Kota Qiang Hao Muralikrishna Narra Vaishnavi Anumula A.V Rao Zanmin Hu Sadanandam Abbagani
• 간행물 정보: 『Journal of Plant Biotechnology』46권 4호, 323~330쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.12.31

영문 초록

The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum- specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the trans-formation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.

목차

Introduction

Materials and Methods

Results

Discussion

Conclusion

키워드

Scoparia dulcis L. Heterologous vectors CaIA plastid vector Inverted repeat region Transplastomic lines