편백의 다신초 유도 및 발근을 통한 식물체 재분화

• 영문명: In vitro regeneration of Chamaecyparis obtusa
• 발행기관: (사)한국식물생명공학회
• 저자명: 김지아(Ji Ah Kim) 이나념(Na-Nyum Lee) 김용욱(Yong Wook Kim)
• 간행물 정보: 『Journal of Plant Biotechnology』46권 4호, 303~309쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.12.31

영문 초록

A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3- butyric acid.

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키워드

Anti-browning Chamaecyparis obtuse Multi- shoot induction