무궁화 품종 ‘난파’의 효율적인 재분화 체계 확립

• 영문명: Establishment of an efficient regeneration system for Hibiscus syriacus ‘Nanpa’
• 발행기관: (사)한국식물생명공학회
• 저자명: 손지희(Ji-Hi Son) 선현진(Hyeon-Jin Sun) 강홍규(Hong-Gyu Kang) 서석철(Seok-Chul Suh) 이효연(Hyo-Yeon Lee)
• 간행물 정보: 『Journal of Plant Biotechnology』46권 4호, 297~302쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.12.31

영문 초록

Hibiscus syriacus L., the national flower of Korea, is a commonly grown ornamental species found in parks, gardens and along roadsides in Korea. This study sought to establish a plant regeneration system of H. syriacus ‘Nanpa’ using mature leaves as an explant. Sterilized mature leaf explants were cultured on modified MS medium with combinations of NAA and BAP at various concentrations for 6 weeks. Among the various media evaluated, modified MS media containing 0.46 mg/L NAA and 0.5 mg/L BAP (CI-1) or 0.92 mg/L NAA and 1 mg/L BAP (CI-4) were the most effective for callus formation. Whitish-yellow calluses were observed on CI-1 medium, while green calluses formed on CI-4 medium. The whitish-yellow callus derived from CI-1 medium showed a higher frequency of shoot induction than the green callus derived from CI-4 on modified MS medium containing 0.5 mg/L BAP. Among the various media evaluated in this study, modified MS medium containing 0.46 mg/L NAA and 0.5 mg/L BAP was the most optimal for shoot- forming callus production. Our findings show that mature leaves of H. syriacus ‘Nanpa’ can be used for in vitro plant regeneration, and the regeneration system described here may be a powerful tool for molecular breeding of H. syriacus ‘Nanpa’ in the future.

목차

서 론

재료 및 방법

결과 및 고찰

결 론

키워드

Callus formation Hibicus syriacus ‘Nanpa’ Mature leaf Molecular breeding Plant regeneration Shoot induction