해당화 열매 성숙단계에 따른 생리활성 및 기능성 물질 변화 분석

• 영문명: Variation in bioactive principles and bioactive compounds of Rosa rugosa fruit during ripening
• 발행기관: (사)한국식물생명공학회
• 저자명: 곽민정(Minjeong Kwak) 엄승희(Seung Hee Eom) 길진수(Jinsu Gil) 김주성(Ju-Sung Kim) 현태경(Tae Kyung Hyun)
• 간행물 정보: 『Journal of Plant Biotechnology』46권 3호, 236~245쪽, 전체 10쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.09.30

영문 초록

Fruit ripening is a genetically programmed process involving a number of biochemical and physiological processes assisted by variations in gene expression and enzyme activities. This process generally affects the phytochemical profile and the bioactive principles in fruits and vegetables. To appraise the variation in bioactive principles of fruits from Rosa rugosa during its ripening process, we analyzed the changes in antioxidant and anti-elastase activities and polyphenolic compounds during the four ripening stages of fruits. Overall, an extract of unripe fruits contained the highest levels of total phenolic and flavonoid contents, radical scavenging activity, reducing power, oxygen radical antioxidant capacity, and elastase inhibitory activity, compared with the extracts of fruits at other stages of ripening. Additionally, we found that the reduction of flavonoid content occurs because of decreased transcriptional levels of genes involved in flavonoid biosynthesis pathway during the ripening process. Based on HPLC analysis, we found that the extract of unripe fruits contained the highest amount of myricetin, caffeic acid, chlorogenic acid, syringic acid, and p-coumaric acid and suggested that the antioxidant and anti-elastase activities of the extract obtained from stage 1, should be mediated by the presence of these compounds. Additionally, we analyzed the interaction sites and patterns between these compounds and elastase using the structure-based molecular docking approach, and suggested that chlorogenic acid strongly interacted with elastase. Together, these findings suggest that the maturity of fruits has profound effects on the pharmaceutical value of R. rugosa.

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키워드

Rosa rugosa Antioxidant Anti-elastase In-silico docking