Small non-coding RNA를 발현하는 형질전환 벼의 환경위해성 평가 방법

• 영문명: Methods for environmental risk assessment of rice transgenic plants expressing small non-coding RNA
• 발행기관: (사)한국식물생명공학회
• 저자명: 진병준() 전현진() 조현민() 이수현() 최철우() 정욱헌() 백동원() 한창덕() 김민철()
• 간행물 정보: 『Journal of Plant Biotechnology』46권 3호, 205~216쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2019.09.30

영문 초록

Since the RNA interference (RNAi) had been discovered in many organisms, small non-coding RNAmediated gene silencing technology, including RNAi have been widely applied to analysis of gene function, as well as crop improvement. Despite the usefulness of RNAi technology, RNAi transgenic crops have various potential environmental risks, including off-target and non-target effects. In this study, we developed methods that can be effectively applied to environmental risk assessment of RNAi transgenic crops and verified these methods in 35S::dsRNAi_eGFP rice transgenic plant we generated. Off-target genes, which can be non-specifically suppressed by the expression of dsRNAi_eGFP, were predicted by using the published web tool, pssRNAit, and verified by comparing their expressions between wild-type (WT) and 35S::dsRNAi_eGFP transgenic rice. Also, we verified the non-target effects of the 35S:: dsRNAi_eGFP plant by evaluating horizontal and vertical transfer of small interfering RNAs (siRNAs) produced in the 35S::dsRNAi_eGFP plant into neighboring WT rice and rhizosphere microorganisms, respectively. Our results suggested that the methods we developed, could be widely applied to various RNAi transgenic crops for their environmental risk assessment.

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키워드

Rice RNA interference Small non-coding RNA Off-target effect Environmental risk assessment