Initiation of embryogenic callus from mature zygotic embryos in Japanese larch (Larix kaempferi)

• 발행기관: (사)한국식물생명공학회
• 저자명: Yong-Wook Kim
• 간행물 정보: 『Journal of Plant Biotechnology』42권 3호, 223~227쪽, 전체 5쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.09.30

영문 초록

Embryogenic callus (EC) was created from mature embryos of Larix kaempferi. With the mature embryos, keeping the culture in dark conditions throughout the experiment (38.2%) seemed to give better results than exposing them to 16 h light (25 μEm -2 S -1 ) for the first week (21.9%). EC was obtained most frequently from Quoirin and Lepoivre (LP) mediums with 1.0 mg/L 4-amino-3,5,6- trichloropicolinic acid (Picloram), plus 1.0 mg/L benzyladenine (BA) (62.8%) or Litvay``s medium (LM) containing 1.0 mg/L p-chlorophenoxyacetic acid (pCPA) plus 1.0 mg/L BA (62.8%) treatment. In both cases, best results were obtained when zygotic embryos were cultured in darkness. As for the effective sucrose concentration on initiation of EC, 29.2 mM sucrose (38.6%) gave the best results.

목차

Introduction

Materials and Methods

Results and discussion

Conclusion

키워드

Embryogenic callus Japanese larch Somatic embryos Zygotic mature embryos