Alteration of plant hormones in transgenic rice (Oryza sativa L.) by overexpression of anti-apoptosis genes during salinity stress

• 발행기관: (사)한국식물생명공학회
• 저자명: Mohammad Ubaidillah Fika Ayu Safitri Sangkyu Lee Gyu-Hwan Park Kyung-Min Kim
• 간행물 정보: 『Journal of Plant Biotechnology』42권 3호, 168~179쪽, 전체 12쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.09.30

영문 초록

We previously identified the rice gene, OsSAP, as an encoder of a highly conserved putative senescenceassociated protein that was shown to have anti-apoptotic activity. To confirm the role of OsSAP in inducing abiotic stress tolerance in rice, we introduced OsSAP and AtBI-1, a plant homologue of Bax inhibitor-1, under the control of the CaMV 35S promoter into the rice genome through Agrobacterium- mediated transformation. The OsSAP transformants showed a similar chlorophyll index after salinity treatments with AtBI-1. Furthermore, we compared the effects of salinity stress on leaves and roots by examining the hormone levels of abscisic acid (ABA), jasmonic acid (JA), gibberellic acid (GA3), and zeatin in transformants compared to the control. With the exception of phytohormones, stress-induced changes in hormone levels putatively related to stress tolerance have not been investigated previously. Hormonal level analysis confirmed the lower rate of stress in the transformants compared to the control. The levels of ABA and JA in OsSAP and AtBI-1 transformants were similar, where stress rates increased after one week and decreased after a two week period of drought; there was a slightly higher accumulation compared to the control. However, a similar trend was not observed for the level of zeatin, as the decrease in the level of zeatin accumulation differed in both OsSAP and AtBI-1 transformants for all genotypes during the early period of salinity stress. The GA3 level was detected under normal conditions, but not under salinity stress.

목차

Introduction

Materials and Methods

Result

Discussion

키워드

Plant hormone Rice response Salinity stress Anti-apoptosis genes OsSAP AtBI-1