Isolation and Characterization of an Anti-listerial Bacteriocin from Leuconostoc lactis SD501

• 영문명: Isolation and Characterization of an Anti-listerial Bacteriocin from Leuconostoc lactis SD501
• 발행기관: 한국축산식품학회
• 저자명: In-Chan Hwang Ju Kyoung Oh Sang Hoon Kim Se-jong Oh Dae-Kyung Kang
• 간행물 정보: 『Food Science of Animal Resources』Korean Journal for Food Science of Animal Resources 제38권 제5호, 1008~1018쪽, 전체 11쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2018.10.31

영문 초록

Although bacteriocins with anti-listerial activity have been isolated from a wide variety of lactic acid bacteria, little is known about those from Leuconostoc lactis, a heterofermentative bacterium that produces diacetyl and exopolysaccharides in dairy foods. In this study, an anti-listerial bacteriocin was isolated from Leuc. lactis SD501 and characterized. It was particularly potent against Listeria monocytogenes and also inhibited Enterococcus faecalis. Anti-listerial activity reached a maximum during the early stationary phase and then decreased gradually. The anti-listerial substance was sensitive to proteinase K and α-chymotrypsin, confirming its proteinaceous nature. Its activity remained stable at pH values ranging from 1 to 10. In addition, it was strongly resistant to high temperatures, retaining its activity even after incubation for 15 min at 121℃. The apparent molecular mass of the partially purified anti-listerial bacteriocin was approximately 7 kDa. The characteristics of the SD501 bacteriocin, including its small molecular size (<10 kDa), strong anti-listerial activity, wide pH stability and good thermostability, indicate its classification as a Class Ⅱa bacteriocin.

목차

Introduction

Materials and Methods

Results and Discussion

Conclusion

키워드

Leuconostoc lactis bacteriocin Listeria monocytogenes