학술논문
Interferon-γ가 치주인대세포의 Collagen 및 Fibronectin의 합성과 Alkaline Phosphatase 활성에 미치는 영향
이용수 0
- 영문명
- Effects of Interferon-γ on collagen and fibronectin synthesis in primary cultured periodontal ligament cells
- 발행기관
- 대한치과교정학회
- 저자명
- 김광석(Gwang-Seok Kim) 성재현(Jae-Hyun Sung) 최제용(Je-Yong Choi) 류현모(Hyun-Mo Ryou)
- 간행물 정보
- 『The Korean Journal of Orthodontics』제23권 제2호, 229~248쪽, 전체 20쪽
- 주제분류
- 의약학 > 기타의약학
- 파일형태
- 발행일자
- 1993.06.30
무료
구매일시로부터 72시간 이내에 다운로드 가능합니다.
이 학술논문 정보는 (주)교보문고와 각 발행기관 사이에 저작물 이용 계약이 체결된 것으로, 교보문고를 통해 제공되고 있습니다.
국문 초록
영문 초록
Interferon-γ has been suggested as a cytokine of connective tissue stabilizer. In addition, it has also been demonstrated that this cytokine inhibited bone remodeling activities of the bone derived cells. In order to illuminate the effects of this cytokine in orthodontic force induced bone remodeling, it was administered to primary cultured periodontal ligament cells which have been known to have some osteoblast like characteristics. Interferon-γ slightly decreased [³H]thymidine incorporation rate without a significant change in the total cellular DNA content up to 1000 U/ml, which meant these doses were not cytotoxic to the cell. Total protein synthesis was not influenced by various concentration of interferon-γ whether it was determined by the [³H]proline incorporation rate or by the Lowry smethod. The effect of interferon-γ on the individual protein was, however, differential, ie, it increased [³H]proline incorporation into the noncollagenous protein marginally, while it decreased [³H]proline incorporation into the collagen, so that it caused dose-dependent suppression of the relative collagen synthesis. On the contrary, the fibronectin synthesis determined by the ELISA was increased by 1000 U/ml of interferon-γ. The differential effects of the interferon-γ on the collagen and fibronectin synthesis exhibited not only their protein level but also the steady state mRNA level. Interferon-γ decreased steady state level of α1(I) procollagen mRNA significantly, while showing no significant changes in the fibronectin mRNA level. In addition to this, it was also found that indomethacin did not affect on the interferon-γ induced collagen decrease in this cell, which meant prostaglandins were not involved in the process of interferon-γ induced collagen decrease. So it can be concluded that the incubation of periodontal ligament cells with 1000 U/ml of interferon-γ for 24 hr showed differential effects on the type I collagen and fibronectin gene expression. The decrease in relative collagen synthesis in the protein level was related with decrease in the steady state level of mRNA, while the increase in the fibronectin synthesis in the protein level was not correlated with the mRNA level.
목차
해당간행물 수록 논문
- 제III급 부정교합자의 안정위시와 /s/ 발음시 혀의 위치에 관한 두부방사선계측학적 연구
- 한국인 8세에서 16세까지 비부외형의 성장 변화
- Elastic tie back 을 이용한 발치공간 폐쇄에 관한 임상적 고려
- 골격성 하악전돌자의 혀와 구강용적의 상관관계에 대한 연구
- 상악매복치의 진단 및 처치
- Interferon-γ가 치주인대세포의 Collagen 및 Fibronectin의 합성과 Alkaline Phosphatase 활성에 미치는 영향
- 재생방법에 따른 교정용 브라켓의 세포독성에 관한 실험적 연구
- 상악 제일대구치의 저항중심에 관한 유한요소법적 분석
- 시간경과에 따른 교정용 braket과 교정선 사이의 마찰력 변화에 관한 연구
- 백서의 전치 이동시 초기 변화에 관한 조직학적 및 자기방사법적 연구
참고문헌
교보eBook 첫 방문을 환영 합니다!
신규가입 혜택 지급이 완료 되었습니다.
바로 사용 가능한 교보e캐시 1,000원 (유효기간 7일)
지금 바로 교보eBook의 다양한 콘텐츠를 이용해 보세요!