Alternative Isoforms of TonEBP with Variable N-termini are Expressed in Mammalian Cells

• 영문명: Alternative Isoforms of TonEBP with Variable N-termini are Expressed in Mammalian Cells
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Hyo Shin Kim Sook Jin Son Seon Nyo Kim Yong Duk Kim Kwang Jin Kim Byeong Hwa Jeon Jin Bong Park Sang
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제11권 제3호, 135~138쪽, 전체 4쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2007.01.01

영문 초록

Hypertonicity imposes a great deal of stress to cells since it causes rise in cellular ionic strength, which can be reduced by the accumulation of compatible osmolytes. TonEBP plays a central role in the cellular accumulation of compatible osmolytes via transcriptional stimulation of membrane transporters and aldose reductase. Alternatively spliced forms of TonEBP mRNA have previously been reported and two of them showed different transcriptional activity. In the present study, isoform-specific antibodies were produced to confirm the translation of the spliced mRNA to protein. TonEBP was immunoprecipitated by using anti-TonEBP antibody and then immunoblotted using anti-TonEBP or isoform specific antibodies to find out the expression profile of TonEBP isoforms in basal or stimulated condition. From these results, we conclude that all TonEBP isoforms are expressed in mammalian cells and their expression patterns are not same in every cells.

키워드

TonEBP Hypertonicity Alternative splicing