p38 MAPK and NF-κB are Required for LPS-Induced RANTES Production in Immortalized Murine Microglia (BV-2)

• 영문명: p38 MAPK and NF-κB are Required for LPS-Induced RANTES Production in Immortalized Murine Microglia (BV-2)
• 발행기관: 대한생리학회-대한약리학회
• 저자명: Sae-Byeol Jang Kweon-Haeng Lee
• 간행물 정보: 『The Korean Journal of Physiology & Pharmacology』제4권 제5호, 339~346쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2000.01.01

영문 초록

Using murine immortalized microglial cells (BV-2), we examined the regulation of RANTES production stimulated by lipopolysaccharide (LPS), focusing on the role of mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB. The result showed that RANTES (regulated upon activation of normal T cell expressed and secreted) was induced at the mRNA and protein levels in a dose- and time-dependent manner in response to LPS. From investigations of second messenger pathways involved in regulating the secretion of RANTES, we found that LPS induced phosphorylation of extracellular signal-regulated kinase (Erk), p38 MAPK and c-Jun-N-terminal kinase (JNK), and activated NF-κB. To determine whether this MAPK phosphorylation is involved in LPS-stimulated RANTES production, we used specific inhibitors for p38 MAPK and Erk, SB 203580 and PD 98059, respectively. LPS-induced RANTES production was reduced approximately 80% at 25μM of SB 203580 treatment. But PD 98059 did not affect RANTES production. Pyrrolidine-dithiocarbamate (PDTC), NF-κB inhibitor, reduced RANTES secretion. These results suggest that LPS-induced RANTES production in microglial cells (BV-2) is mainly mediated by the coordination of p38 MAPK and NF-κB cascade.

키워드

Microglia BV-2 RANTES LPS MAPK NF-κB