Regulatory Action of ß-adrenergic Agonist and 8-bromocyclic AMP on Calcium Currents in the Unfertilized Mouse Eggs

• 영문명: Regulatory Action of ß-adrenergic Agonist and 8-bromocyclic AMP on Calcium Currents in the Unfertilized Mouse Eggs
• 발행기관: 대한생리학회
• 저자명: Haan, Jae-Hee Cheong, Seung-Jin Kim, Yang-Mi Park, Choon-Ok Hong, Seong-Geun
• 간행물 정보: 『대한생리학회지』제27권 제2호, 175~184쪽, 전체 10쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1993.12.31

영문 초록

There are many report suggesting that influx and intracellular calcium concentration ([Ca²⁺]_i) are related to cell signalling in various cells. However, it has not been reported that calcium channel activation is affected by the substances involved in signal transduction pathways in the mouse eggs. In this study, the effects of isoprenaline (ISP) and cyclic AMP on calcium influx through calcium channels were investigated to show their relationship with the signal transduction process in unfertilized mouse eggs. Using whole cell voltage clamp techniques, calcium currents, elicited by the depolarizing pulses of 300 ms duration (from -50 mV to 50 mV in 10 mV increments) from a holding potential of -80 mV, were recorded. The current-voltage (I-V) relation of calcium currents was shown to be bell-shaped; the current began to activate at -50 mV and reached its maximum (-1.33±0.16 nA: mean±S.E., n=7) at -10 mV, then decayed at around 50 mV. Calcium currents were fully activated within 7 ms ~ 20 ms and completely inactivated 200 ms after onset of the step pulse. ISP within the concentration ranges of 10^-8 M ~ 10^-4 M dose-dependently increased the amplitude calcium current. The permeable cyclic AMP analogue,8-bromocyclic AMP, also increased its maximal amplitude by 46ft at 10^-5 M, while protein kinase inhibitor (PKI), which is known to inhibit 0.02 phosphorylating units of cyclic AMP-dependent protein kinase (PKA) per microgram decreased calcium currents. Currents recorded in the presence of PKI were resistant to increase by the application of 10^-5 M. Also, PKI inhibited the calcium current increase elicited by ISP treatment. These results suggest that β-adrenergic regulation of the calcium channel is mediated by the cAMP-dependent protein kinase. This signal transduction pathway might play a role in regulating [Ca²⁺]_i, level due to the increase of calcium influx in mouse eggs.

키워드

Mouse eggs Calcium channel Isoprenaline 8-bromocyclic AMP Protein kinase inhibitor Whole cell voltage clamp technique