Immunocytochemical Study on the Translocation Mechanism of Glucose Transporters by Insulin

• 영문명: Immunocytochemical Study on the Translocation Mechanism of Glucose Transporters by Insulin
• 발행기관: 대한생리학회
• 저자명: Hah, Jong-Sik Kim, Ku-Ja
• 간행물 정보: 『대한생리학회지』제27권 제2호, 123~138쪽, 전체 16쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1993.12.31

영문 초록

The mechanism of insulin action to increase glucose transport is attributed to glucose transporter translocation from intracellular storage pools to the plasma membrane in insulin-sensitive cells. The present study was designed to visualize the redistribution of the glucose transporter by means of an immunogold labelling method. Our data clearly show that glucose transporter molecules were visible by this method. According to the method this distribution of glucose transporters between cell surface and intracellular pool was different in adipocytes. The glucose transporter molecules were randomly distributed at the cell surface whereas the molecules at LDM were farmed as clusters. By insulin treatment the number of homogeneous random particles increased at the cell surface whereas the cluster forms decreased at the intracellular storage pools. It suggests that the active molecules needed to be evenly distributed far effective function and that the inactive molecules in storage pools gathered and termed clusters until being transferred to the plasma membrane.

키워드

Glucose transporter GLUT1 GLUT4 Insulin Translocation Immunogold labelling