토끼에서 태자를 통하지 않은 양수내 Li+의 이동

• 영문명: Extrafetal Transfer of Li⁺ in Amniotic Fluid of Pregnant Rabbits
• 발행기관: 대한생리학회
• 저자명: 김영제(Kim, Young-Jae) 호원경(Ho, Won-Kyung) 성호경(Sung, Ho-Kyung)
• 간행물 정보: 『대한생리학회지』제24권 제1호, 27~38쪽, 전체 12쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1990.06.30

영문 초록

The extrafetal transfer of Li⁺ in amniotic fluid was studied in 45 pregnant rabbits. LiCl solution was administered either intravenously to mother or directly into the amniotic sac and monitored the appearance and disappearance of Li⁺ in the amniotic fluid, then calculated the transfer rate of Li⁺ of extrafetal origin. To study the transplacental Li⁺ transfer, a solution of 150 mM LiCl was infused continuously via maternal vein (initial dose: 0.7 mmol/kg, maintaining dose: 0.03 mmol/kg/min) and the Li⁺ concentration was measured in maternal blood and amniotic fluid after 60 and 120 minutes of infusion. Change in the volume of aminotic fluid was determined by Congo red dilution method at the same time. Effects of duration of gestation was not considered in this study. Extrafetal transport of Li⁺ into the amniotic fluid was estimated by comparing the Li⁺ concentration and volume of amniotic fluid determined before and after ligating the placental vessels. Extrafetal Li⁺ transport from the amniotic fluid was determined by observing the time dependent disappearance of Li⁺ and Congo red in amniotic fluid after injecting 0.5 ml solution of 15 mM or 90 mM LiCl and 50 mg/ml Congo red. Following are the results obtained: 1) During infusion of LiCl through maternal vein the ratio of the aminotic Li⁺/maternal plasma Li⁺ increased significantly along with the increment of fetal weight. 2) The volume of amniotic fluid of larger fetuses than 20.5 gm increased significantly during administration of LiCl while that of smaller fetuses did not change. 3) After umbilical cord ligation the Li⁺ concentration of amniotic fluid of larger fetuses than 20.5 gm was decreased to 59.9±10.3% and 56.9±42.9% (mean±S.D.) of those of control group after 60 and 120 minutes of LiCl infusion respectively. In amniotic fluid of smaller fetuses than 20.5 gm, there was no significant difference between control and ligation groups. 4) The disappearance rate of Congo red in the amniotic fluid was 45.2±8.2%/hr. 5) The disappearance rate of Li⁺ after intraamniotic injection of LiCl depended on the amount injected. On injecting 7.5 μmol LiCl, Li⁺ disappeared rapidly from the amniotic fluid and the rates after 60 min and 90 min were 97.0±2.8, 98.5±2.0% respectively. On injecting 45 μmol LiCl, the rates were 56.0±15.4, 78.9±14.5% at 60 and 90 min. 6) From the above results it was concluded: a) Li⁺ transfer into the amniotic fluid increased along with the fetal growth and one half of Li⁺ influx is through the extrafetal route even after the maturation of fetal kidney. b) One half of the Li⁺ transfer from the amniotic fluid was through swallowing of fetus, while the remaining half was transfered rapidly through amniotic membrane, which was concentration limited.

키워드

Lithium ion Amniotic fluid Extrafetal route