Vanadate가 가토신피질 Na-K-ATPase활성에 미치는 영향

• 영문명: Effect of Vanadate on Na-K-ATPase Activity of Rabbit Kidney Cortex
• 발행기관: 대한생리학회
• 저자명: 우종렬(Woo, Jong-Ryeol) 한복기(Han, Bok-Ki) 이상호(Lee, Sang-Ho)
• 간행물 정보: 『대한생리학회지』제17권 제2호, 161~168쪽, 전체 8쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1983.12.31

영문 초록

Studies on the effects of vanadate for Na-K-ATPase activity were carried out with rabbit renal cortex. 1) Na-K-ATPase activity was inhibited with the concentrations of vanadate in incubation medium. The vanadate concentration at which activity was inhibited by 50%(ID₅₀) was 10^-6M and Hill coefficient was 1.00. 2) The fractional inhibition by constant concentration of vanadate decreased with increasing enzyme concentration. 3) Increasing K⁺ and Na⁺ concentrations in incubation medium diminished the ability to inhibit Na-K-ATPase by vanadate whereas increasing K⁺ and Mg²⁺ concentrations potentiated the inhibition of Na-K-ATPase by vanadate. 4) Vanadate didn t inhibit Na-K-ATPase at pH 6.6. Increasing pH potentiated the inhibition of Na-K-ATPase activity. 5) Vanadate inhibited Na-K-ATPase activity reversibly in all range of concentrations in dilution experiment. These results show that vanadate inhibits Na-K-ATPase activity with interacting at KE² state reversibly.