Dopamine이 흰쥐 자궁의 자발적 수축에 미치는 영향

• 영문명: Role of Dopamine upon Spontaneous Contraction of Isolated Rat Uterus in Diestrus State
• 발행기관: 대한생리학회
• 저자명: 박형진(Park, Hyoung-Jin) 심여림(Sim, Yeo-Rim) 조양혁(Jo, Yang-Hyeok)
• 간행물 정보: 『대한생리학회지』제17권 제2호, 103~108쪽, 전체 6쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 1983.12.31

영문 초록

This experiment was undertaken to see whether dopamine has any effect on a uterine function and whether the uterus has a dopamine receptor. We used 14 female rats in the diestrus state which was identified by a vaginal smear. Under ether anesthesia, 3 pieces(1 cm length) from each side of the uterus were dissected out and mounted in 3 tissue chambers (4 cm diameter, 10 cm height) that contained Krebs-Ringer solution. The solution was continuously aerated with 95% O₂ containing 5% CO₂ and kept 37℃ consistantly during the whole experimental period. The spontaneous contractile activity of the isolated uterus was recorded using a force transducer. After a recovery period of 15 min in the chamber, the following experiments were carried out. In 7 rats, each piece of the uterus was received dopamine at concentrations of 10^-4, 10^-5 or 10^-6 M for 10 min and then followed by domperidone at a concentration of 10^-5 M. In another 7 rats, each piece was received domperidone, a specific peripheral dopamine receptor antagonist, was administered at a concentration of 10^-5 M for 5 min prior to dopamine at concentrations of 10^-4, 10^-5, or 10^-6 M. Dopamine inhibited the spontaneous uterine contraction dose-dependently (r=0.99, p<.01). The inhibited contractility by dopomine was significantly (P<.05) resumed by post-treatment of domperidone. Pre-treatment of domperidone also blocked significantly(p<.05) the inhibitory effect of dopamine. It is concluded from these results that dopamine has inhibitory role upon the spontaneous uterine contraction of the rat in the diestrus state and domperidone antagonized the inhibitory effect of dopamine. These results suggest strongly that dopamine may exert the inhibitory effect via the dopamine receptor in the rat uterus.