학술논문
Titanium disc에 도포한 부착단백질이 사람 치은 조섬유세포의 부착 및 증식에 미치는 영향
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- 영문명
- 발행기관
- 대한치과이식임플란트학회
- 저자명
- 최정원 강윤선 이근우
- 간행물 정보
- 『Journal of Dental Implant Research』제13권 제1호, 82~104쪽, 전체 23쪽
- 주제분류
- 의약학 > 의학일반
- 파일형태
- 발행일자
- 1993.12.31
5,560원
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국문 초록
영문 초록
Endosseous dental implants are interfaced with bone, connective tissue, and epithelium when implanted
into the jaw bone. The soft tissue interface including connective tissue and epithelium is one
of the most critical factors in the determination of implant maintenance and prognosis. The connecdtive
tissue interface not only forms the attachment apparatus, but also contributes to the formation of
healthy peri-implant tissue by prevention of the epithelial downgrowth. There are two ways to promote
connective tissue fibroblasts adhesiveness to implanted biomaterials, one is to control the surface
roughness of the biomaterial and the other is to coat the biomaterial with attachment proteins. Between
them, coat the biomaterials with attachment proteins is denounced. Fibronectin and type I collagen
are the only attachment proteins studied to titanium biomaterials for evaluation of their effectiveness,
and the results were not united. And it is difficult to evaluate the exact effect of the attachment
proteins due to experimental difficulties and methods of application. So the comparative analytical
studies of the effect of attachment proteins under controlled condition are needed.
The purpose of this in vitro study was to evaluate the adhesiveness and proliferation of human
gingival fibroblasts to attachment protein-coated and non-coated endosseous titanium biomaterials.
Well-known attachment proteins were used, they were type I collagen, type IV collagen, fibronectin,
laminin, and vitronectin. Each attachment proteins applied onto the commercially pure titanium discs.
In this study, the protein-coated and non-coated titanium discs were classified as each groups. Human
gingival fibroblasts cultured onto each groups. After 30,60, 180 mins incubation time, unattached
cells counted with Coulter counter for evaluation of the adhesiveness of human gingival fibroblasts.
The configurations of attached human gingival fibroblasts were done by SEM observation. To confirm
the focal contact areas, actin cytochemical and vinculin immunofluorescent staning were done after
3, 24 hours of incubation. To evaluate the effect of attachment proteins on the proliferation of human
gingival fibroblasts, 3H-thymidine were added after 72 hours incubation. The results were as follows.
1. Adhesiveness of human gingival fibroblasts were best in fibronectin-coated group among groups
(p>0.05). Then laminin, vitronectin, and type IV collagen groups were better, and there were
no statistically significant difference between type I collagen group and control (p<0.05).
2. Fibronectin and type IV collagen groups showed well spread human gingival fibroblasts in SEM
observation.
3. Fibronectin group showed most dense and regular arrangement of actin filaments among groups.
4. Fibronectin group showed most distinct vinculin patches along the cytoplasmic process among
groups.
5. In the proliferation of human gingival fibroblasts, there were no stasistically significant difference
among groups (p<0.05).
On the bases of these findings, it is strongly suggest that fibronectin coating at the neck portion
of titanium biomaterial promotes adhesiveness of human gingival fibroblasts and suggest the possibility
of healthy implant maintenance. Clinical application of the attachment proteins are. further studied
and comparative analysis of attachment proteins applied on implant biomaterials against epithelial
cells are needed.
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