Chlorpromazine이 백서 해마에서 전기경련 충격에 의한 MAPK 타이로신 인산화에 미치는 영향

• 영문명: The Effects of Chlorpromazine on the Tyrosine Phosphorylation of MAPK Induced by Electroconvulsive Shock in Rat Hippocampus
• 발행기관: 대한신경정신의학회
• 저자명: 정성훈 김상욱 강웅구 전송희 주연호 박주배 김용식
• 간행물 정보: 『신경정신의학』제36권 제2호, 368~375쪽, 전체 8쪽
• 주제분류: 의약학 > 정신과학
• 파일형태: PDF
• 발행일자: 1997.03.30

영문 초록

Object：In order to examine the interaction mechanisms of electroconvulsive shock(ECS) and antipsychotic drug at the level of molecular biology, we observed the effect of chlorpromazine pretreatment on the activation of mitogen activated protein kinase(MAPK) induced by electroconvulsive shock(ECS) in rat hippocampus Method：Male Sprague-Dawley rats were divided into 2 groups. To the experimental group chlorpromazine(20mg/kg) was given intraperitoneally, and to the control distilled water was given instead. Thirty minutes later, ECS was given and the hippocampus was dissected out 2 minutes thereafter. Immunoblotting with antiphosphotyrosine antibody was carried out, and the signal intensity at 42kDa band was quantitized using densitometer. The obtained result was compared by student t-test between the experimental and the control group. The absolute amount of MAPK was measured by immunoblotting with anti-MAPK antibody. Result：The tyrosine phosphorylation of MAPK reached peak at 2 minutes after ECS. However, in the chlorpromazine pre-treated group, the peak level of MAPK tyrosine phosphorylation was significantly attenuated(t＝-3.12, df＝14, p＝0.008) compared to the control. In contrast to this, the absolute amount of MAPK did not differ between the pre-treated and the control group. Conclusion：Chlorpromazine attenuated the tyrosine phosphorylation of MAPK by ECS. This finding seems to be related to the fact that chlorpromazine pre-treatment changed the c-fos expression by ECS in rat brain. Antipsychotic drug and ECS might interact at the level of MAPK signal transduction system, and this might explained the observed synergistic effect of two treatment modality.

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키워드

Chlorpromazine Electroconvulsive shock Immunoblot Mitogen Activated Protein Kinase Tyrosine phosphorylation