Glutamate 와 Glycine 이 휜쥐 뇌의 N-Methyl-D-Aspartate 수용체에 대한 [3H]MK-801 결합반응에 미치는 영향

• 영문명: Effects of Glutamate and Glycine on the Binding of [3H]MK-801 to the N-Methyl-D-Aspartate Receptor in Rat Brain
• 발행기관: 대한신경정신의학회
• 저자명: 정영인 김영훈 김명정
• 간행물 정보: 『신경정신의학』제32권 제4호, 545~553쪽, 전체 9쪽
• 주제분류: 의약학 > 정신과학
• 파일형태: PDF
• 발행일자: 1993.07.30

영문 초록

In an attempt to investigate the characteristics of N-methyl-D-aspartate (NM D A ) authors receptor which is expected to play an important role in neuropsychiatric field recently, the author performed receptor binding assay using [3H]MK-801[( + ) —5 —methyl-10, 11-dihydro- 5H-dibenzo[a, d]-cycloheptene-5, 10-imine maleate], which is known to selectively antagonize the NM D A receptor in noncompetitive fashion, in order to observe the effects of glycine and glutamate on [3H]MK-801 binding to rat brain membranes. The results are summarized as follows ; 1) Glutamate and glycine enhanced the binding of [3H]MK-801 to rat brain membranes. 2) In inhibition experiment of [3H]MK-801 binding to rat brain membranes by unlabeled ( + ) — M K —801 and Mg++, glutamate and glycine did not affect IC 50 of unlabeled ( + ) _ MK- 801, but reduced that of Mg++. 3) Glutamate and glycine did not affect Bmax of [3H]MK-801 to rat brain membranes, but decreased Kd value of [3H]MK-801. In conclusion, these results indicate that glutamate and glycine activate the N M D A receptor through distinct recognition sites respectively which may be allosterically coupled, and the the enhancement of [3H]MK-801 binding produced by these amino acids may result from the increase of affinity for but not the change in Bmax of [3H]MK-801.

목차

서 론

재료 및 방법

결 과

고 찰

요 약

References

키워드

N-methyl-D-aspartate (NMDA) receptor Glutamate Glycine, [3H]MK-801 Neurodegenerative disease Schizophrenia