Graphene accelerates osteoblast attachment and biomineralization

• 영문명: Graphene accelerates osteoblast attachment and biomineralization
• 발행기관: 한국탄소학회
• 저자명: Jia Ren,Xiaogang Zhang,Yao Chen
• 간행물 정보: 『Carbon Letters』제22권, 42~47쪽, 전체 6쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2017.04.01

영문 초록

In this paper, the in vitro biocompatibility of graphene film (GF) with osteoblasts was evaluated through cell adhesion, viability, alkaline phosphatase activity, F-actin and vinculin expressions, versus graphite paper as a reference material. The results showed that MG-63 cells exhibited stronger cell adhesion, better proliferation and viability on GF, and osteoblasts cultured on GF exhibited vinculin expression throughout the cell body. The rougher and wrinkled surface morphology, higher elastic modulus and easy out-of-plane deformation associated with GF were considered to promote cell adhesion. Also, the biomineralization of GF was assessed by soaking in simulated body fluid, and the GF exhibited enhanced mineralization ability in terms of mineral deposition, which almost pervaded the entire GF surface. Our results suggest that graphene promotes cell adhesion, activity and the formation of bonelike apatite. This research is expected to facilitate a better understanding of graphene-cell interactions and potential applications of graphene as a promising toughening nanofiller in bioceramics used in load-bearing implants.

목차

1. Introduction

2. Experimental

3. Results and Discussion

4. Conclusions

References

키워드

graphene film,osteoblast,adhesion,viability,mineralization