서로 다른 추출방식이 블랙커런드 추출물의 항산화 및 항염 효능에 미치는 영향

• 영문명: Antioxidant and Anti-inflammatory Effects of Blackcurrant(Ribes nigrum L) Extracted with Different Methods
• 발행기관: 충북대학교 생활과학연구소
• 저자명: 한인화(In Hwa Han) 조진아(Jin Ah Cho) 윤은성(Eun Seung Yoon) 이수진(Su Jin Lee) 전지향(Ji Hyang Jeon) 정해진(Hae Jin Jeong) 홍지수(Ji Su Hong) 김경아(Kyung Ah Kim)
• 간행물 정보: 『생활과학연구논총』제28권 제2호, 27~37쪽, 전체 11쪽
• 주제분류: 자연과학 > 생활/식품과학
• 파일형태: PDF
• 발행일자: 2024.08.31

국문 초록

Background/Objectives: Blackcurrants are well-known for their high content of antioxidants and their anti-inflammatory properties, both of which contribute to various health benefits. This study aims to investigate the antioxidant and anti-inflammatory activities of 80% ethanol extracts of blackcurrant (BCEs), extracted using different methods. Methods: Blackcurrant powder was extracted by two different methods: stirring (BCE-S) and ultrasound (BCE-U). Antioxidant efficacy was assessed by measuring the total phenol content, total flavonoid content, and DPPH radical scavenging ability of the BCEs. Cell viability was determined using the WST assay in Raw 264.7 cells while the anti-inflammatory effect was evaluated by observing the expression levels of COX-2, an inflammation-related protein, through western blot analysis. Results: BCE-S and BCE-U did not exhibit significantly different total phenol content and DPPH radical scavenging ability. However, the total flavonoid content was higher in BCE-S than in BCE-U. Cytotoxicity evaluation revealed that both extracts did not show cytotoxicity at all treatment concentrations. The protein level of COX-2, increased by LPS treatment, was reduced in BCE-U but not in BCE-S. Despite the difference in total flavonoid content, there was no difference in antioxidant efficacy between the two extracts. However, BCE-U exhibited superior anti-inflammatory efficacy, suggesting variations occurred in the extract functionality based on the extraction method. Conclusion/Implications: These results provide fundamental data for establishing an efficient extraction method that can be tailored to obtain specific functionalities.

목차

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 결과 및 논의

Ⅳ. 결론 및 제언

참고문헌

키워드

Blackcurrant Extraction methods Antioxidant activity Anti-inflammatory activity