Exploring amygdala structural changes and signaling pathways in postmortem brains: consequences of long-term methamphetamine addiction

• 영문명: Exploring amygdala structural changes and signaling pathways in postmortem brains: consequences of long-term methamphetamine addiction
• 발행기관: 대한해부학회
• 저자명: Zahra Azimzadeh Samareh Omidvari Somayeh Niknazar Saeed Vafaei-Nezhad Navid Ahmady Roozbahany Mohammad-Amin Abdollahifar Foozhan Tahmasebinia Gholam-Reza Mahmoudiasl Hojjat Allah Abbaszadeh Shahram Darabi
• 간행물 정보: 『Anatomy and Cell Biology』Vol.57(1), 70~84쪽, 전체 15쪽
• 주제분류: 의약학 > 의학일반
• 파일형태: PDF
• 발행일자: 2024.03.30

영문 초록

Methamphetamine (METH) can potentially disrupt neurotransmitters activities in the central nervous system (CNS) and cause neurotoxicity through various pathways. These pathways include increased production of reactive nitrogen and oxygen species, hypothermia, and induction of mitochondrial apoptosis. In this study, we investigated the long-term effects of METH addiction on the structural changes in the amygdala of postmortem human brains and the involvement of the brain- cAMP response element-binding protein/brain-derived neurotrophic factor (CREB/BDNF) and Akt-1/GSK3 signaling pathways. We examined ten male postmortem brains, comparing control subjects with chronic METH users, using immunohistochemistry, real-time polymerase chain reaction (to measure levels of CREB, BDNF, Akt-1, GSK3, and tumor necrosis factor-α [TNF-α]), Tunnel assay, stereology, and assays for reactive oxygen species (ROS), glutathione disulfide (GSSG), and glutathione peroxidase (GPX). The findings revealed that METH significantly reduced the expression of BDNF, CREB, Akt-1, and GPX while increasing the levels of GSSG, ROS, RIPK3, GSK3, and TNF-α. Furthermore, METH-induced inflammation and neurodegeneration in the amygdala, with ROS production mediated by the CREB/BDNF and Akt-1/GSK3 signaling pathways.

목차

Introduction

Materials and Methods

Results

Discussion

ORCID

Author Contributions

Conflicts of Interest

Funding

Acknowledgements

References

키워드

Methamphetamine Brain-derived neurotrophic factor cAMP response element-binding protein Amygdala