신생아 분변에서 분리한 Enterococcus faecalis의 내산성 및 내담즙산성 비교

• 영문명: Comparison of Acid and Bile Tolerances for Enterococcus faecalis isolated from Infant Feces
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 김성준(Seongjun Kim) 오세종(Sejong Oh)
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』Current Topics in Lactic Acid Bacteria and Probiotics 4권 2호, 14~18쪽, 전체 5쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2016.12.30

영문 초록

In this study, we investigated the probiotic candidates of Enterococcus faecalis isolated from infant feces. Fecal samples were inoculated on modified MRS (pH 5.4) agar and Blood-liver containing antibiotics agar. Total 10 isolates were identified through Gram stain, KOH test, catalase test, and 16S rDNA analysis. And these isolates compared to resistance against artificial gastric juice (pH 2.5, 1,000 unit pepsin) and bile salts (0.3% Oxgall). Especially, strain SJ10 survives at a level of 7.84 log CFU/ mL under 0.3% bile salts. Strains SJ1, SJ6, SJ7 and SJ10 were found to be the least acid resistance with a viable cells (SJ1: 6.97 log CFU/mL, SJ6: 7.19 log CFU/mL, SJ7: 7.02 log CFU/mL and SJ 10: 7.07 log CFU/mL, respectively). Further in vitro and in vivo studies should be evaluated with these isolates before claiming probiotic characteristics.

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키워드

Enterococcus faecalis probiotics bile tolerance acid tolerance