Lactobacillus plantarum L67의 항알러지 활성

• 영문명: Suppressive Effect of Lactobacillus plantarum L67 on Allergic Disease Risk Factors
• 발행기관: 한국유산균프로바이오틱스학회
• 저자명: 신미영(Miyoung Shin) 송수연(Sooyeon Song) 고종호(Jongho Koh) 김광현(Kwang-Hyun Kim) 오세종(Sejong Oh)
• 간행물 정보: 『Current Topics in Lactic Acid Bacteria and Probiotics』Current Topics in Lactic Acid Bacteria and Probiotics 4권 2호, 38~47쪽, 전체 10쪽
• 주제분류: 농수해양 > 식품과학
• 파일형태: PDF
• 발행일자: 2016.12.30

영문 초록

In this study, we found that the glycoprotein (18 kDa) isolated from Lactobacillus plantarum L67 (L67 protein) inhibits allergy-related inflammatory mediators in BPA treated rat basophilic leukemia 2H3 (RBL-2H3) cells and primary cultured splenocytes. First, we measured the levels of ERK, p38, and AP-1 by Western blotting and we evaluated the expression of IL-1β, IL-6, and IL-10 cytokines by real-time quantitative PCR. Finally, we evaluated the applicability of L. plantarum L67 as a yogurt starter culture by measuring pH, enumeration of bacteria, and sensory scores. The results of this study shows that L67 protein inhibits the phosphorylation of ERK and p38 MAPK, as well as the activation of AP-1 (c-Fos and c-Jun). With inflammationrelated factors (Th2-related cytokines), L67 protein inhibits BPA-induced IL-1β, IL-6, and IL-10 activity. Bisphenol A stimulates MAPK signaling, which induces increased GATA-3 expression, and GATA-3 induces the differentiation of Th2 cells and secretion of Th2-related cytokines. Our findings showed that L67 protein may regulate the activation of GATA3 and T-bet through the induction of MAPK signaling, and it relieves the Th1/Th2 cytokine profile imbalance. Taken together, these findings demonstrate that the L. plantarum L67 strain is a potential food supplement and starter culture, that could help in the prevention of Th2-related immune disorders.

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allergy bisphenol A Lactobacillus plantarum L67 probiotics