Development of EST-SSRs and Assessment of Genetic Diversity in Germplasm of the Finger Millet, Eleusine coracana (L.) Gaertn.

• 발행기관: 한국작물학회
• 저자명: Xiaohan Wang 이명철(Myung Chul Lee) 최유미(Yu-Mi Choi) 김성훈(Seong-Hoon Kim) 한세희(Seahee Han) Kebede Taye Desta 윤혜명(Hye-myeong Yoon) 이윤정(Yoonjung Lee) 오미애(Miae Oh) 이정윤(Jung Yoon Yi) 신명재(Myoung-Jae Shin) 김경민(Kyung-Min Kim)
• 간행물 정보: 『한국작물학회지』제66권 제4호, 443~451쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2021.12.30

영문 초록

Finger millet (Eleusine coracana) is widely cultivated in tropical regions worldwide owing to its high nutritional value. Finger millet is more tolerant against biotic and abiotic stresses such as pests, drought, and salt than other millet crops; therefore, it was proposed as a candidate crop to adapt to climate change in Korea. In 2019, we used expressed sequence tag simple sequence repeat (EST-SSR) markers to evaluate the genetic diversity and structure of 102 finger millet accessions from two geographical regions (Africa and South Asia) to identify appropriate accessions and enhance crop diversity in Korea. In total, 40 primers produced 116 alleles, ranging in size from 135 to 457 bp, with a mean polymorphism information content (PIC) of 0.18225. Polymorphism was detected among the 40 primers, and 13 primers were found to have PIC values > 0.3. Principal coordinate and phylogenetic analyses, based on the combined data of both markers, grouped the finger millet accessions according to their respective collection areas.Therefore, the 102 accessions were classified into two groups, one from Asia and the other from Africa. We have conducted an in-depth study on the finger millet landrace pedigree. By sorting out and using the molecular characteristics of each pedigree, it will be useful for the management and accession identification of the plant resource. The novel SSR markers developed in this study will aid in future genetic analyses of E. coracana.

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MATERIALS AND METHODS

RESULTS

DISCUSSION

ACKOWLEDGEMENTS

REFERENCES

키워드

phylogenetic analysis population structure selfing plants