Immunomodulatory effect of splenectomy in lung cancer mouse xenograft models receiving radiation therapy

• 발행기관: 대한방사선종양학회
• 저자명: Yeeun Kim Changhoon Choi Jee Hyun Park Won-Gyun Ahn Sung-Won Shin Shin-Yeong Kim Jae Myoung Noh
• 간행물 정보: 『대한방사선종양학회지』제40권 제1호, 53~65쪽, 전체 13쪽
• 주제분류: 의약학 > 종양학
• 파일형태: PDF
• 발행일자: 2022.03.30

영문 초록

Purpose: This study aims to investigate the effect of splenectomy on radiation-mediated growth inhibition and immune modulation in lung cancer xenograft models. Materials and Methods: Human non-small cell lung cancer H1299 cells and murine Lewis lung carcinoma LL/2-luc cells were injected into the right hind leg of BALB/c-nude mice and C57BL/6 mice, respectively. Splenectomy or sham operation was performed prior to tumor cell injection or before and after irradiation during tumor growth. Irradiation was delivered with 2-3 fractions of 6 Gy X-ray using a linear accelerator. Flow cytometry analysis was performed for immune cell profiling. Results: Splenectomy prior to tumor injection or at early stage inhibited growth of LL/2-luc tumors but not that of H1299 tumors; however, it did not enhance the antitumor effect of radiation regardless of intervention timing. Flow cytometry analysis showed monocytic myeloid-derived suppressor cells (MDSCs) and activated CD8+ T cells increased after irradiation in the tumors of splenectomized mice, compared to those of sham-operated mice. Administration of anti-PD-1 (programmed death-1) antibodies improved the ability of splenectomy to attenuate the growth of irradiated tumors. Conclusion: Splenectomy has paradoxical effects on radiation-induced tumor growth inhibition, depending on tumor types and intervention timing, but it has an immune-modulating effect when combined with radiation.

목차

Introduction

Materials and Methods

Results

Discussion

Conflict of Interest

Acknowledgements

References

키워드

Radiotherapy Myeloid-derived suppressor cells Splenectomy Lung neoplasms