알루미늄 내성과 민감성 보리의 빠른 screening과 원형질막 H^+-ATPase의 발현

• 영문명: A Rapid Screening for Aluminum-tolerant and -sensitive in Barley (Hordeum vulgare L.) and Plasma Membrane H^+-ATPase Expression
• 발행기관: 한국작물학회
• 저자명: 김현성(Hyun-Sung Kim) 안성주(Sung-Ju Ahn 오정민
• 간행물 정보: 『한국작물학회지』제56권 제1호, 72~79쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2011.03.30

영문 초록

Here we report a simple screening system using hematoxylin staining (HS) of the root apex. It allowed rapid classification into different aluminum (Al)tolerance from 65 cultivars within one week. Using this system, we selected the most Al-tolerant (Jayae-2) and -sensitive (Pum-2) barley (Hordeum vulgare L.) The results show that the different responses in Al-induced growth inhibition,Al accumulation and expression of plasma membrane (PM)H^+-ATPase in root apices of selected two cultivars. It showed strongly Al-induced growth inhibition in a dosedependant manner only in Pum-2 but not in Jayae-2. Aluminum accumulation in root apices (10 mm) was significantly higher in Pum-2 only. The H^+-ATPase expression of PM vesicles by western blotting was decreased in Pum-2 but not in Jayae-2 treated with 20 μM Al for 24 h. These finding indicate to screen from our system is rapid and reliable and to sustain the expression of PM H^+-ATPase at translational level is an important role in root growth as affected by Al.

목차

재료 및 방법

결과 및 고찰

적요

사사

인용문헌

키워드

aluminum barley H^+-ATPase plasma membrane rapid screening