복숭아 NGS 분석에 의한 다형성 SSR 마커 개발과 활용

• 영문명: Anticipated Polymorphic SSRs and Their Application Based on Next Generation Sequencing of Prunus Persica
• 발행기관: 한국육종학회
• 저자명: 김정선(Jung Sun Kim) 구윤숙(Yoon Suk Ku) 박신기(Sin-Gi Park) 김세희(Se Hee Kim) 박현우(Hyun Woo Park) 원소윤(So Youn Won)
• 간행물 정보: 『한국육종학회지』Vol.53 No.4, 350~372쪽, 전체 23쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2021.12.01

영문 초록

Prunus persica “Mihong” cultivar is a domesticated white peach that was generated from the crossing between “Yumyeong” and“Chiyomaru” cultivars in the Republic of Korea in 1995. We launched “Mihong” genome sequencing in 2018 and “Mihong” reached to 200scaffold and 241 Mb sequences using long-read sequencing and Hi-C technology. F1 populations of ”Kawanakajima Hakuto,” “Mihong,”“Changhowon Hwangdo,” and “Yumi” were developed in NIHHS. These four cultivars were sequenced and assembled using the SOAPdenovoversion 2.04. First, we surveyed the SSRs in “Mihong” assembly sequences and extracted the ±300 bp flanking sequences containing SSRs. Second, the assembly sequences of three cultivars were aligned and mapped against “Mihong” ±300 bp flanking sequences using BLASTn(version 2.2.29+). We anticipated the differential length in SSRs among the four cultivars. We sorted the primers with a standard deviationover 4.5 (STEV > 4.5) among the four cultivars. In addition, we surveyed the primers having difference in over 10 bp with “KawanakajimaHakuto” and “Mihong” for polymorphic markers in the mapping population. All primer pairs were designed to generate amplicons of 150-200bp in coating SSR regions using primer3 (version 3-2.2.3). We selected 260 SSR markers with a physical distance of average per 1 Mb. These SSR markers accounted for 74% polymorphism in the four genotypes. Finally, a F1 population of “Kawanakajima Hakuto” and “Mihong”covered 884.5 cM with 465 SNPs and 86 SSRs and this genetic map matched correctly to the HI-C pseudomolecule of P. persica.

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peach Prunus persica SSR markers genetic map