Proteome Approach as a Tool for the Efficient Separation of Seed Storage Proteins from Buckwheat

• 발행기관: 한국작물학회
• 저자명: Seong-Woo Cho Soo-Jeong Kwon Swapan Kumar Roy Sun-Hee Woo
• 간행물 정보: 『한국작물학회지』제60권 제1호, 29~32쪽, 전체 4쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.03.30

영문 초록

Two-dimensional electrophoresis (2-DE) was executed to separate the seed storage proteins from the buckwheat. The proteins extracted from the whole seed proteins were better separated and observed in the use of lysis buffer. Using this method, the highly reproducible isoelectric focusing (IEF) can be obtained from polyacrylamide gels, and IEF from the polyacrylamide gel at all the possible pH range (5.0-8.0) was more easily separated than IPG (immobilized pH gradient) gels. The polyacrylamide gels in the first dimension in 2-DE was used to separate and identify a number of whole seed proteins in the proteome analysis. In this new apparatus using 2-DE, 27cm in length of plate coated with polyacrylamide gel was used and the experiment was further investigated under the various conditions.

목차

Results and Discussion

Results and Discussion

키워드

Buckwheat 2-DE Efficient Separation Method Seed Storage Protein Proteome Approach