Proteomics를 이용한 재배 환경에 따른 콩 종실 단백질 발현 양상 비교

• 영문명: Analysis of Protein Function and Comparison of Protein Expression of Different Environment in Soybean using Proteomics Techniques
• 발행기관: 한국작물학회
• 저자명: 조성우(Seong-Woo Cho) 김태선(Tae-Sun Kim) 권수정(Soo-Jeong Kwon) Swapan Kumar Roy 이철원(Chul-Won Lee) 김홍식(Hong-Sig Kim) 우선희(Sun-Hee Woo)
• 간행물 정보: 『한국작물학회지』제60권 제1호, 33~40쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.03.30

영문 초록

Soybean is very useful crop to supply vegetable protein for human. Supply of soybean is increased because it has useful ingredient. Recently, cultivation of soybean in paddy field is increasing due to the increase of rice stockpile in Korea. Hence, in this study, expression of protein was identified regarding different environment for cultivation to investigate the effect of different environment on protein expression. Two-dimensional electrophoresis was performed to investigate the expression of protein using image analysis program to measure degree of protein expression in numerical value. Hannam-kong, Beakcheon-Kong, Hwangkeum-Kong, and Danwon-Kong were used as plant material. 2-DE combined with image analysis revealed that each degree of protein expression of Hannam-Kong and Hwangkeum-Kong in upland field was higher than degree of protein expression in paddy field. However, in case of Beackcheon-Kong, the phenomenon was opposite. In Danwon-kong, the degree of protein expression was not different between up-land field and paddy field. To this end, major protein spots were not different between paddy field and upland field among all cultivars. It could be suggested that protein expression is not severely different by various environment, but different environment affects degree of protein expression.

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키워드

Soybean Proteomics 2-DE Seed Storage Protein Protein expression