Proteomics를 이용한 등숙기 차이에 따른 콩 종실 저장단백질 발현양상 비교 분석

• 영문명: Analysis of Protein Function and Comparison on Expression of Protein in Taekwang During Maturation using Proteomic Techniques
• 발행기관: 한국작물학회
• 저자명: 조성우(Seong-Woo Cho) 김태선(Tae-Sun Kim) 권수정(Soo-Jeong Kwon) Swapan Kumar Roy 이철원(Chul-Won Lee) 김홍식(Hong-Sig Kim) 우선희(Sun-Hee Woo)
• 간행물 정보: 『한국작물학회지』제60권 제1호, 41~46쪽, 전체 6쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2015.03.30

영문 초록

In the present study, different expression of protein from Taekwang was revealed by 2-DE, and expressions of protein on each week after flowering was investigated. After analysis of expression of protein, MALDI-TOF was executed to identify expected protein function. Results revealed that there were three patterns of expression of protein during the maturing. The first pattern was that proteins were gradually expressed as up-regulation from 1 week to 6 week. The second pattern was that proteins were expressed gradually from 1 week to 5 week and then it started down-regulation in 6 week. The last pattern was that proteins were gradually as up-regulation from 1 week to 3 week and then down-regulation until 6 week. This phenomenon suggests that young stage has more protein related to correspondence mechanism against disease and growth and then maturing stage has more expression of protein related to storage protein. In MALDI-TOF analysis, p24 oleosin isoform A protein was identified that relates oleosin which is synthetic product in oil body. This protein spot increased gradually until 5 week and then decreased after 5 week. It explained that the protein is active until maturing stage to protect oil in seed and then its activity has gradually degraded. This result may be expected that a protein, related to growth of a seed has increased until maturing and then a seed fills up with a storage protein.

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키워드

Soybean Seed Storage Protein 2-DE MALDI-TOF Protein expression