Dysfunction of Retinal Cell and Optic Nerve by Continuous Cerebroventricular Infusion of Glucosamine

• 발행기관: 대한검안학회
• 저자명: Younghwa Kim Hwa-Young Lee Hoo Jae Hann Innoc Han Seikwan Oh Rumin Jun Kyu-Ryong Choi
• 간행물 정보: 『Annals of optometry and contact lens』Vol.3 No.3, 1~28쪽, 전체 28쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2004.12.30

영문 초록

It has been known that excessive glucose flux through the hexosamine biosynthetic pathway and accumulation of glucosamine could reduce the neuroprotective effect of insulin or directly affect survival mechanism in retinal neurons. We have investigated the effect of glucosamine on the retinal cells by continuous infusion into the cerebroventricle, using osmotic mini-pumps to avoid peripheral effects. Continuous intra cerebroventricular (i.c.v) infusion of glucosamine at a rate of 10μⅼ/hr(0.1 ㎛ol) for 7 days resulted in morphological changes in the optic nerve at the electron microscopic level as well as morphological changes in the retina at light microscopic level. Retina was cut into 10㎛ thick on a cryostat and sections were collected on gelatin-coated slides. Retinal sections were immunostained for the morphological changes of astrocytes. GFAP immunoreactivity appeared not only in the Muller cells but also in many of the radial processes of Muller cells. The ultrathin (50 nm) transverse sections of the optic nerve (2 mm behind the eyeball) were transferred to grids and examined with an electron microscope. The optic nerve showed deformed axons and slight lamellar separation of myelin sheath after continuous infusion with glucosamine. Interestingly, vacuoles were observed in the deformed axons and retinal layers were folded and detached. These results suggest that glucosamine plays a major role in induction of morphological dysfunction in retina and optic nerves.

목차

INTRODUCTION

EXPERIMENTAL PROCEDURE

RESULTS

DISCUSSION

REFERENCES

키워드

glucosamine retinal cell osmotic minipump O-glycosylation GFAP vimentin