Establishment of in vitro Root Cultures and Analysis of Secondary Metabolites in Indian Ginseng - Withania somnifera

• 발행기관: 한국자원식물학회
• 저자명: Neha G. Wasnik Mahalakshmi. Muthusamy Savitha Chellappan Veena Vaidhyanathan Ramakrishna Pulla Kalaiselvi Senthil Deok Chun Yang
• 간행물 정보: 『한국자원식물학회지』제22권 제6호, 584~591쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2009.12.31

영문 초록

Adventitious root culture was established in the Jawahar variety of Withania somnifera using MS basal medium supplemented with 0.5 (mg/l) IAA and 2.0 (mg/l) IBA. Root tips from germinated seedlings, MS0 maintained plants and adventitious roots were maintained in suspension medium (1/2 MS basal medium supplemented with 3% sucrose) for a period of 1 to 6 months. The weight gain in roots was noted and the withanolides were extracted from the dry roots using solvents petroleum ether, 50% ethanol and chloroform. The withanolides in the chloroform fractions of all root samples analyzed were compared using thin layer chromatographic analysis. Withanolide content in adventitious root sample was found to be superior compared to other roots at any given point of time during the 6month growth period.HPLC analysis of in vitro adventitious roots showed the presence of a new compound.

목차

INTRODUCTION

MATERIAL AND METHODS

RESULT AND DISCUSSION

LITERATURE CITED

키워드

Adventitious roots germinated roots MS0 withanolides TLC