한국 대표 밀 품종의 미숙배 채취 시기별 조직배양 효율

• 영문명: The Effects of Harvesting Time on the Efficiency of Tissue Culture Used Immature Embryos from Korean Wheat Cultivars
• 발행기관: 한국육종학회
• 저자명: 최창현(Changhyun Choi) 손재한(Jae-Han Son) 박진희(Jin Hee Park) 김경민(Kyeong-Min Kim) 김경훈(Kyeong-Hoon Kim) 정한용(Han-Yong Jung) 손지영(Ji-Young Shon) 박태일(Tae-Il Park) 강천식(Chon-Sik Kang)
• 간행물 정보: 『한국육종학회지』Vol.52 No.4, 382~388쪽, 전체 7쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2020.12.30

영문 초록

The regeneration rate of plantlets cultivated via tissue culture is an important factor for wheat transformation. Similar to othermonocotyledons, the most efficient tissue culture materials for wheat are immature embryos. However, stable year-round production of immatureembryos is not possible in the field where various stress factors co-exist. In this study, we investigated the generation and subsequent plantletincident rates of callus induced from immature embryos obtained from different sowing times in 2020 and compared these among wheatcultivars. We found that the rates of regeneration and plantlet incidence obtained using immature embryos of the Ariheuk cultivar were higherthan those of other domestic cultivars, and that the tissue culture efficiency was similar to that of Bobwhite, which has been establishedas a cultivar with excellent transformation efficiency. Furthermore, the Baekkang cultivar showed high tissue culture efficiency only whensown from early to mid-March, whereas Keumkang showed higher tissue cultivation efficiency only by sowing in mid- and late February. Among the five cultivars assessed in this study, Jopum showed the lowest tissue culture efficiency. It is anticipated that the findings of thisstudy will contribute to enhancing the transformation efficiency of domestic wheat varieties.

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키워드

wheat tissue culture immature embryo regeneration Korean wheat cultivar