4년근 미성숙 인삼열매 분획물의 약리학적 특성 및 치매 마우스 모델에서 인지능 개선효과

• 영문명: Pharmacological Properties of the Four-year Unripe Ginseng Berry and the Enhancing Effect on Learning and Memory in Aβ42-induced Alzheimer Mouse Model
• 발행기관: 대한약학회
• 저자명: 안정원(Jeong Won Ahn) 장수길(Su Kil Jang) 김현수(Hyun Soo Kim) 조보람(Boram Jo) 성은아(Eun Ah Sung) 김승태(Seung Tae Kim) 최은경(Ehn-Kyoung Choi) 김윤배(Yun-Bae Kim) 박희용(Hee Yong Park) 주성수(Seong Soo Joo)
• 간행물 정보: 『약학회지』제64권 제2호(2020년), 109~123쪽, 전체 15쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2020.04.30

영문 초록

In the present study, we aimed to determine whether the processed 4-year ginseng berry extract (SGF) had pharmacological properties with improving learning and memory in an Aβ42-induced Alzheimer’s mouse model. Passive avoidance test (PAT) and Morris water-maze test (MWMT) were performed after the administration with SGF, and assays (acetylcholine, ACh and Aβ) in the brain lysates were followed along with the glial fibrillary acidic protein (GFAP) detection for the brain damage. Acetylcholinesterase (AChE) activity and the gene expression (choline acetyltransferase (ChAT), vesicular acetylcholine transporter (VAChT) and brain-derived neurotrophic factor (BDNF)) in N2a cells was further analyzed using Ellman’s and qPCR assays, respectively. Results demonstrated that SGF contained a high amount of 3 active ginsenosides (Rg3, Rg5 and F4) and significantly improved PAT and MWMT compared to Aβ42-induced mouse model. Interestingly, AChE activity in the brain lysates was significantly reduced, whereas ACh amounts was escalated in SGF groups. In addition, ChAT, VAChT and BDNF mRNAs were significantly upregulated in the presence of a single Rg3, Rg5 and F4 as well as SGF. Taken together, these findings clearly suggest that SGF can participate in alleviating AD pathogenesis by preventing the brain damage and increasing ACh production which are primitive requirements for a therapeutic candidate of Alzheimer’s disease.

목차

서 론(Introduction)

실험방법(Experimental Methods)

결과 및 고찰(Results and Discussion)

결 론(Conclusion)

감사의 말씀(Acknowledgment)

Conflict of Interest

References

키워드

ginseng berry passive avoidance test Morris water-maze test acetylcholine choline acetyltransferase ginsenoside