치주염 동물모델을 이용한 해조류의 항염증 효과

• 영문명: Anti-inflammatory Effects of Seaweeds Using Periodontitic Animal Model
• 발행기관: 한국구강보건과학회
• 저자명: 윤현서(Hyun-Seo Yoon) 박충무(Chung-Mu Park)
• 간행물 정보: 『한국구강보건과학회지』제8권 제1호, 38~43쪽, 전체 6쪽
• 주제분류: 의약학 > 기타의약학
• 파일형태: PDF
• 발행일자: 2020.03.30

영문 초록

Objectives: This study investigated the anti-inflammatory activity of four kinds of seaweeds (Ecklonia cava, Saccharina japonica, Undaria pinnatifida, and Gelidium amansii) in the RAW 264.7 cell line and in a mouse model of periodontitis. Methods: The anti-inflammatory activity of prepared ethanol extracts of the seaweeds was screened in the RAW 264.7 cell line. A diet containing 5% of each seaweed was administered to the mice for 2 weeks, followed by lipopolysaccharide (LPS) injection into the periodontal tissue to induce inflammation. Nitric oxide (NO) production was analyzed by the Griess reaction and expression of inflammatory marker proteins was determined by western blotting. Results: All four ethanol extracts inhibited NO production in a concentration-dependent manner without cytotoxicity. In addition, all four seaweed extracts inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 expressions in periodontal tissues of LPS-injected C57BL/6 mice. The mouse group administered U. pinnatifida ethanol extract (UPEE) showed the strongest anti-inflammatory response. Conclusions: The four seaweeds, E. cava, S. japonica, U. pinnatifida, and G. amansii, all exhibited anti-inflammatory activity in both the RAW 264.7 cell line and the LPS-induced periodontitic mouse model. UPEE showed the most potent anti-inflammatory activity. These seaweeds might be promising candidates for alleviating periodontal inflammation. Further research is needed to confirm their effectiveness as preventive agents for periodontal disease.

목차

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 연구결과

Ⅳ. 고찰 및 결론

REFERENCES

키워드

Periodontitis Seaweed Undaria pinnatifida Anti-inflammatory Periodontitic Animal Model