A study on the inflammatory response induced by LPS of the Arthrospira platensis ethanol extract

• 발행기관: 한국응용과학기술학회 (구.한국유화학회)
• 저자명: Shi Jie Zhang Jae-Chan Yang Bo-Ae Kim
• 간행물 정보: 『한국응용과학기술학회지』한국응용과학기술학회지 제36권 제3호, 966~974쪽, 전체 9쪽
• 주제분류: 공학 > 화학공학
• 파일형태: PDF
• 발행일자: 2019.09.30

영문 초록

Arthrospira platensis has been reported to contain a variety of substances such as phycocyanin, β-carotene, vitamin E and other carotenoids. In this study, zebrafish were treated with indoor cultivation spirulina ethanol extracts(ICAE) to determine toxicity(coagulation rate, hatching rate, heart rate). We used the DCFH-DA staining method to detect the effect of reactive oxygen species(ROS) generation on lipopolysaccharide(LPS)-induced zebrafish embryos ROS various concentrations(0.01, 0.05, 0.1, 0.5mg/ml) of ICAE. Cell toxicity was measured by WST-1 assay on RAW 264.7 macrophage cell line. Also, measured the inhibitory effect of nitric oxide(NO) and prostaglandin E₂(PGE₂) production in RAW264.7 macrophages induced by LPS at various concentrations of ICAE. The results of embryo coagulation rate, hatching rate, heart rate of zebrafish at various(0.01, 0.05, 0.1mg/ml) of ICAE was no toxicity. The ICAE treated group had an inhibitory effect on NO and PGE₂ production compared and decreased with concentration. The results of this study ethanol extract of Arthrospira platensis has an anti-inflammatory effect and suggest that is worthy of use cosmetics for skin protection.

목차

1. INTRODUCTION

2. MATERIALS AND METHODS

3. RESULTS AND DISCUSSION

4. CONCLUSION

키워드

Arthrospira platensis zebrafish anti-inflammation toxicity DCFH-DA reactive oxygen species nitric oxide prostaglandin E₂