Herbicide‐resistant Transgenic Mongolian Bentgrass (Agrostis mongolica Roshev.) obtained by Agrobacterium‐mediated Transformation

• 발행기관: 한국육종학회
• 저자명: Enkhchimeg Vanjildorj Tae‐ Woong Bae In‐ Ja Song Kyung‐ Moon Kim Yong‐ Pyo Lim Hyo‐ Yeon Lee
• 간행물 정보: 『한국육종학회지』Vol.40 No.2, 128~135쪽, 전체 8쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 2008.06.30

영문 초록

Herbicide resistance is the most common trait being tested and thus herbicide‐resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide‐resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium‐mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S‐bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.

목차

INTRODUCTION

RESULTS AND DISCULLSION

키워드

Agrobacterium‐ mediated transformation Agrostis mongolica Roshev. Herbicide resistance Regenerable and reproducible callus type