명아주 추출물의 항산화 효과에 의한 배양 인체피부섬유모세포에 대한 카드뮴 손상방어

• 영문명: Protection of Chenopodium album var. centrorubrum Extract by Oxidative Effect in Cultured Human Skin Fibroblasts Damaged by Cadmium
• 발행기관: 인간식물환경학회
• 저자명: 손영우(Young Woo Son) 최유선(Yu Sun Choi)
• 간행물 정보: 『인간식물환경학회지(JPPE)』제15권 제3호, 155~161쪽, 전체 7쪽
• 주제분류: 자연과학 > 자연과학일반
• 파일형태: PDF
• 발행일자: 2012.06.30

영문 초록

To evaluate the cytotoxicity of cadmium(Cd), an environmental pollutant, and the protective effect of Chenopodium album var. centrorubrum(CAC) extract on the Cd-induced cytotoxicity in cultured human skin fibroblasts(Detroit 551). Cultured Detroit 551 cells were treated or pretreated with cadmiun chloride(CdCl₂) or CAC extract. In this study, CdCl₂ significantly decreased cell viability compared with control, and XTT₅₀ or XTT₅₀ value(midcytotoxicity value, MCV) was determined at a concentration of 41.1μM or 43.1μM. The MCV of CdCl₂ was highly toxic by the criteria of Borenfreund and Puerner. While, CdCl₂-induced cytotoxicity was prevented by superoxide dismutase(SOD), an antioxidative enzyme. In the protective effect of CAC extract on CdCl₂-induced cytotoxicity, CAC extract prevented CdCl₂-induced cytotoxicity by the significant increase of cell viability decreased by CdCl₂-induced cytotoxicity. And also, CAC extract showed antioxidative effects such as electron donating activity(EDA) and inhibitory activity of lipid peroxidation(LP). From above the results, it is suggested that CdCl₂ was highly toxic to cultured Detroit 551 cells and CAC extract was effective in the blocking CdCl₂-induced cytotoxicity by the antioxidative effects such as EDA and inhibitory activity of LP. In conclusion, the physical activity of natural component such as CAC extract may be a useful resource for preventing diseases mediated by reactive oxygen species(ROS) such as heavy metal toxication, stroke or dementia.

목차

Abstract

Ⅰ. 서론

Ⅱ. 연구방법

Ⅲ. 결과 및 고찰

Ⅳ. 적요

Ⅴ. 인용문헌

키워드

Lipid peroxidation Midcytotoxicity value Physical activity Superoxide dismutase