KB(kenacid blue) 시험을 이용한 광독성(phototoxicity) 대체 in vitro 시험 평가 연구

• 영문명: Kenacid blue assay as an in vitro alternative method for phototoxicity evaluation using human fibroblast
• 발행기관: 한국예방수의학회
• 저자명: 이종권(Jong Kwon Lee) 이은희(Eun Hee Lee) 홍진태(Jin Tae Hong) 김형수(Hyung Soo Kim) 김주환(Ju Hwan Kim) 박기숙(Ki Sook Park) 조대현(Dae Hyun Cho) 이선희(Sun Hee Lee) 이영순(Yong Soon Lee)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.24, No.2, 97~103쪽, 전체 7쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 2000.06.30

영문 초록

This study was conducted to assess a prospective altemative method as replacements for in vivo phototoxicity test. The human fibroblasts were exposed to several phototoxic chemicals(promethazine, neutral red, chlortetracycline, amiodarone, bithionol, 8-methoxypsoralen) and a non-phototoxic substance, ammonium laureth sulfate and irradiated with 5J/㎠ of UVA. The cell viability was measured by KB(kenacid blue)assay. The phototoxic potential of test chemicals in the KB PT(phototoxicity test) was assessed by determining the PIF(photoirritancy Factor) by using a cut-off value of 5. The KB PT responses of most chemicals showed a close agreement with in vivo response except chlorotetracycline. There was a relatively good agreement between in vitro KB assay and in vivo data. These results suggest that KB assay using fibroblast could be used to predict the phototoxicity of the chemicals.

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키워드

Phototoxicity KB UVA Fibroblast