우결핵 병리조직으로부터의 우형결핵균의 분리 및 신속동정 방법

• 영문명: Detection of mycobacterium bovis in the pathological lesions of slaughtered dairy cattles using modified culture medium and polymerase chain reaction
• 발행기관: 한국예방수의학회
• 저자명: 배길한(Gill Han Bai) 황현순(Hyun Soon Hwang) 손봉환(Bong Whan Shon)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.18, No.3, 183~190쪽, 전체 8쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1994.11.30

영문 초록

Twenty-seven specimens were sampled from the pathological lesions of the thirteen dairy cattle slaughtered owing to the positive tuberculin reaction. The conventional smear microscopy and the culture on BM medium were compared with the polymerase chain reaction(PCR) technique for the detection of the causative organism of bovine tuberculosis in the sample specimens. On the basis of nucleotide sequence of IS1081 and mtp40 each pair of oligonucleotides was used as primers for the PCR amplification to target 300bp and 396bp respectively. The oligonucleotide primers from IS1081 prodcued positive signals for M. bovis in the amplified reactions while the primer mtp40 did not make PCR product because of its specificity to M. tuberculosis. Of the 27 sample specimens 19(70.4%) were detected as positive by both of the concentrated smear microscopy and the culture on BM media, but 6 specimens were confirmed as positive only by culture. Thus the total cultrue positive sample were 25(92.6%). PCR using IS1081 primers detected 26(96.3%) of these sample as positive for M. bovis. From one PCR negative but the cultrue positive sample M. nonchromogenicum was isolated. Results of the PCR and the culture showed that M. bovis was successfully detected by our suggested methods from the pathological lesions of dairy cattle slaughtered.

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재료 및 방법

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참고문헌

키워드

Mycobaterium bovis PCR BM medium