계태아뇨막강액을 첨가한 계태아 섬유아단층세포에서의 newcstle disease virus의 증식성

• 영문명: Propagation of newcastle disease virus in chicken embryo fibroblast monolayer supplemented with chicken embryo allantoic fluid
• 발행기관: 한국예방수의학회
• 저자명: 윤경진(Kyoung Jin Yoon) 전윤성(Yun Seong Jeon)
• 간행물 정보: 『Korean Journal of Veterinary Public Health』Vol.11, No.2, 27~34쪽, 전체 8쪽
• 주제분류: 의약학 > 면역학
• 파일형태: PDF
• 발행일자: 1987.11.30

영문 초록

Effect of chicken embryo allantoic fluid on the propagation of NDV in CEF culture was studied. Physico-chemical characteristics of chicken embryo allantoic fluid were also investigated. The experimental results were summarized as following: 1. The propagation of NDV(B₁) in CEF culture was promoted by the supplementation of chicken embryo allantoic fluid to the culture medium. 1) Log10 TCID50 0.1ml of NDV(B₁) propagated in embryonated egg was 9.0±1.0 in the CEAF supplemented group and 1.3±0.3 in the control group. 2) Log₂ HA titers of NDV(B₁) propagated in CEF cultures were 7.5±0.5(released and cell-bound virus), 4.0±1.0(released virus) and 8.0±1.0(cell-bound virus) in the CEAF supplemented group. But those of control groups were 4.5±0.5, 0.0, and 4.5±0.5 respectively. 3) Log10 EID50 /0.1ml of NDV(B₁) propagted in CEF culture was 9.3 in the CEAF supplemented group and 3.8 in the control group. 2. Chicken embryo allantoic fluids harvested from 10, 11, or 12-day-old embryonated eggs were suitable for the propagation of NBV(B₁) in CEF culture. 3. The amount of chicken embryo allantoic tluid suitable for the propagation of NDV(B₁) in CEF culture was 5~10%.

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