밀 耐凍性 染色體置換系統에서 光合成의 光沮害現象 差異

• 영문명: Differences in photoinhibition of photosynthesis in wheat chromosome substitution lines for freezing resistance
• 발행기관: 한국육종학회
• 저자명: Jong Un Chun(千鍾殷) In Ho Jeong(鄭仁浩) 마릴린 그리피스(Marilyn Griffith)
• 간행물 정보: 『한국육종학회지』Vol.29 No.3, 359~367쪽, 전체 9쪽
• 주제분류: 농수해양 > 기타농수해양
• 파일형태: PDF
• 발행일자: 1997.09.30

영문 초록

In order to evaluate the relationship between low temperature-induced photoinhibition and winter survival in wheat, seeds of 20 chromosome substitution lines and their parents, Chinese Spring and Cheyenne were germinated at 20℃ and were transferred to 5/2℃ and 20/16℃ growth chambers to obtain cold-acclimated (CA) and nonacclimated (NA) plants, respectively. For photoinhibition and recovery experiments, 7 to 8cm long leaf portions were placed on moist filter paper and placed in trays at 5℃ cold room with 1,100 μmol m⁻² s⁻¹ PPFD. Room temperature chlorophyll fluorescence was measured with a PAM system after 50 minute dark adaptation. Instantaneous Fo, Fv, and Fm were determined and the Fv/Fm, was calculated. Fv/Fm of CA plants were reduced from 0.81 in control leaves to 0.33 following photoinhibition of 8h and the CVs were increased from 1% to 19%. Fv/Fm of NA plants were reduced from 0.82 in control leaves to 0.39 in leaves exposed to 5℃ for 6h, and the CVs were increased from 0.5% to 15%. Thus, photoinhibition of CA plants was less than that of NA plants. Fv/Fm of freezing tolerant Cheyenne grown under either CA and NA conditions, were higher than those of freezing-sensitive Chinese Spring. Chinese Spring lines substituted with Cheyenne chromosome 5(5A, 5B and 5D) showed relatively higher resistance to photoinhibition. A series of studies suggests that the chromosomes of homologous group 5 of Cheyenne have major genes controlling freezing tolerance, expression of antifreeze proteins and factors resistant to low temperature-induced photoinhibition. Winter survival in wheat was positively correlated with resistance to photoinhibition of PSⅡ and significantly fitted by multple linear regression(R²=0.75∗∗). Also, winter survival in wheat was positively correlated with resistance to photoinhibition of PSⅡ and expression of antifreeze proteins, and significantly fitted by multple linear regression (R²=0.76∗∗). Variable chlorophyll fluorescence measured by Fv/Fm has been found to be very useful in evaluating relative levels of freezing tolerance in wheat.

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ABSTRACT

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材料 및 方法

結果 및 考察

摘要

引用文獻

키워드

Photoinhibition Winter survival Chromosome substitution line Fv/Fm Antifrepze protein Chlorophyll fluorescence